Reporter

Part:BBa_J52008:Design

Designed by: Monika Ciglic   Group: iGEM06_Slovenia   (2006-10-14)


luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 547


Design Notes

Rluc is cloned in BioBrick vector pSB1AK3. We had no problems with making this part.

Source

Our source for Renilla luciferase was vector phRL-TK from Promega.

References