Part:BBa_J45993

Minimal stationary phase osmY promoter

BBa_J45993 is a stationary phase promoter derived from the promoter that controls transcription of osmY in Escherichia coli Yim-1992, Yim-1994. BBa_J45993 consists of only 57 base pairs encompassing the -35 and -10 promoter regions \cite{Yim:J-Bacteriol:1994}.

Usage and Biology

Expression of osmY is dependent on σ^S in vivo Hengge-Aronis-1993. Schellhorn et al. previously demonstrated that an osmY::lacZ fusion generated the highest transcriptional signal in stationary phase as compared to nine other σ^S-dependent promoter-lacZ fusions Schellhorn-1998, Vijayakumar-2004. In addition, the osmY::lacZ fusion generated only a small transcriptional signal during exponential growth.

<biblio>

1. Yim-1994 pmid=8282684
2. Yim-1992 pmid=1317380
3. Hengge-Aronis-1993 pmid=8416901
4. Vijaykumar-2004 pmid=15576800
5. Schellhorn-1998 pmid=9829938

</biblio>

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]