Generator
BOG
Part:BBa_J45250:Experience
Designed by: André Green II Group: iGEM2006_MIT (2006-10-28)
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Applications of BBa_J45250
Stationary phase banana odor production from the exogenous precursor isoamyl alcohol.
User Reviews
UNIQ7f98ce23952a4f09-partinfo-00000000-QINU
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The stationary phase banana odor generator (BBa_J45250) produces a banana odor in culture when supplemented with isoamyl alcohol. As expected, the stationary phase banana odor generator produced very little isoamyl acetate at low cell densities but its isoamyl acetate production increased with cell density. |
UNIQ7f98ce23952a4f09-partinfo-00000003-QINU
Characterization
Transcriptional control of banana odor enzyme generator
Growth dependent banana odor production
To demonstrate growth phase dependent banana odor production, we compared the behavior of constitutive and stationary phase dependent banana odor generators (A and B, respectively). We measured isoamyl acetate concentrations of cultures of the constitutive and stationary phase banana odor generators at different culture cell densities (OD600nm) (C). As expected, the stationary phase banana odor generator produced very little isoamyl acetate at low cell densities but its isoamyl acetate production increased with cell density. By comparison, the constitutive banana odor generator produced more isoamyl acetate at lower cell densities than the stationary phase banana odor generator. To visually aid comparison of the two odor generators, an empirical fit to the data for each device is shown.
To demonstrate growth phase dependent banana odor production, we compared the behavior of constitutive and stationary phase dependent banana odor generators (A and B, respectively). We measured isoamyl acetate concentrations of cultures of the constitutive and stationary phase banana odor generators at different culture cell densities (OD600nm) (C). As expected, the stationary phase banana odor generator produced very little isoamyl acetate at low cell densities but its isoamyl acetate production increased with cell density. By comparison, the constitutive banana odor generator produced more isoamyl acetate at lower cell densities than the stationary phase banana odor generator. To visually aid comparison of the two odor generators, an empirical fit to the data for each device is shown.