Part:BBa_J435605:Design

pOSIP-KO (KanR, 186)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 1125
  Illegal EcoRI site found at 1
  Illegal XbaI site found at 28
  Illegal XbaI site found at 1397
  Illegal XbaI site found at 4823
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1
  Illegal NheI site found at 3312
  Illegal NheI site found at 5166
  Illegal SpeI site found at 1126
  Illegal PstI site found at 1140
  Illegal NotI site found at 1133
  Illegal NotI site found at 4358
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1
  Illegal BglII site found at 469
  Illegal BamHI site found at 22
  Illegal XhoI site found at 1152
  Illegal XhoI site found at 5081
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 1126
  Illegal EcoRI site found at 1
  Illegal XbaI site found at 28
  Illegal XbaI site found at 1397
  Illegal XbaI site found at 4823
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1
  Illegal XbaI site found at 28
  Illegal XbaI site found at 1397
  Illegal XbaI site found at 4823
  Illegal SpeI site found at 1126
  Illegal PstI site found at 1140
  Illegal AgeI site found at 1343
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 357
  

Design Notes

https://freegenes.github.io/genes/BBF10K_000492.html

One-step cloning and chromosomal integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. 10.1021/sb400021j PubMed 24050148

Source

https://freegenes.github.io/genes/BBF10K_000492.html

One-step cloning and chromosomal integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. 10.1021/sb400021j PubMed 24050148