Part:BBa_J435393

DF_TEVcut_His_Stop_TZ

A TEV protease recognition domain, HIS tag, and stop codon. Defined as a DF part.

Note: Possible misidentification. Flanking sites on FreeGenes appear to be wrong, possibly incorrect BBF10K number identified.

FLANKED 5' fusion site: AGGT, 3' fusion site: CGCT

Source organism: N/A, Target organism: Escherichia coli

Citation: https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit#gid=1530011293

ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_003459.html

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 76
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 76
  Illegal NotI site found at 218
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 76
  Illegal BamHI site found at 70
  Illegal XhoI site found at 227
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 76
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 76
  
• 1000
  COMPATIBLE WITH RFC[1000]