Composite

Part:BBa_J435320:Design

Designed by: Open Bioeconomy   Group: FreeGenes   (2023-05-08)


AE_lacZ pDest


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 250
    Illegal XbaI site found at 223
    Illegal PstI site found at 211
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 250
    Illegal PstI site found at 211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 250
    Illegal BamHI site found at 229
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 250
    Illegal XbaI site found at 223
    Illegal PstI site found at 211
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 250
    Illegal XbaI site found at 223
    Illegal PstI site found at 211
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 607
    Illegal BsaI.rc site found at 2


Design Notes

Unknown


Source

Source organism: N/A, Target organism: Escherichia coli


Citation: https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit#gid=1530011293

Author: Open Bioeconomy Lab

ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_003375.html

References