Part:BBa_J435209

Sc-pTDH3

S. cerevisiae TDH3 promoter using standard Freegenes syntax

Source organism: S. cerevisiae, Target organism: yeast

Citation: https://pubmed.ncbi.nlm.nih.gov/7737504/

ORG: Open Yeast Collection, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_000134.html

Documentation of the part by PTSH-Taiwan 2023

This is a potent yeast expression promoter derived from the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene, also known as TDH3 or GAPDH. Research has revealed that the promoter's activity significantly decreases when glycerol or xylose is used as the carbon source or when exposed to high temperatures (42°C). Oxygen conditions have a negligible impact.

Regarding the protein, the promoter originates from the GAPDH peptide, which plays a role in glycolysis and gluconeogenesis. This protein exists as a tetramer and facilitates the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, primarily located in the cytoplasm and cell wall. Antimicrobial peptides derived from GAPDH are produced by S. cerevisiae and exhibit activity against a broad spectrum of yeast and bacteria commonly found in the context of winemaking.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]