DNA

Part:BBa_J435117:Design

Designed by: Vinoo Selvarajah   Group: FreeGenes   (2023-05-23)


pOSIP-KO (KanR, 186)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1125
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 28
    Illegal XbaI site found at 1397
    Illegal XbaI site found at 4823
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 3312
    Illegal NheI site found at 5166
    Illegal SpeI site found at 1126
    Illegal PstI site found at 1140
    Illegal NotI site found at 1133
    Illegal NotI site found at 4358
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 469
    Illegal BamHI site found at 22
    Illegal XhoI site found at 1152
    Illegal XhoI site found at 5081
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1126
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 28
    Illegal XbaI site found at 1397
    Illegal XbaI site found at 4823
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 28
    Illegal XbaI site found at 1397
    Illegal XbaI site found at 4823
    Illegal SpeI site found at 1126
    Illegal PstI site found at 1140
    Illegal AgeI site found at 1343
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 357


Design Notes

Unknown


Source

One-Step Cloning and Chromosomal Integration of DNA

https://freegenes.github.io/genes/BBF10K_000492.html

One-step cloning and chromosomal integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. 10.1021/sb400021j PubMed 24050148

References