Plasmid

Part:BBa_J435114:Design

Designed by: Drew Endy, Keith Shearwin   Group: FreeGenes   (2023-05-23)


pE-FLP (AmpR Low-copy)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 837
    Illegal SpeI site found at 1155
    Illegal SpeI site found at 2573
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 837
    Illegal SpeI site found at 1155
    Illegal SpeI site found at 2573
    Illegal NotI site found at 2892
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 837
    Illegal BglII site found at 25
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 837
    Illegal SpeI site found at 1155
    Illegal SpeI site found at 2573
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 837
    Illegal SpeI site found at 1155
    Illegal SpeI site found at 2573
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3538


Design Notes

Unknown


Source

One-step cloning and chromosomal integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. 10.1021/sb400021j PubMed 24050148

References