Coding

Part:BBa_J428063:Design

Designed by: Vinoo Selvarajah   Group: 2021 Engineering Committee   (2022-06-10)


Nickases Cas9n D10A


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 790
    Illegal PstI site found at 2212
    Illegal PstI site found at 2416
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 790
    Illegal PstI site found at 2212
    Illegal PstI site found at 2416
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 790
    Illegal PstI site found at 2212
    Illegal PstI site found at 2416
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 790
    Illegal PstI site found at 2212
    Illegal PstI site found at 2416
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
    Illegal NgoMIV site found at 1078
    Illegal NgoMIV site found at 2182
    Illegal NgoMIV site found at 2255
    Illegal NgoMIV site found at 2740
    Illegal NgoMIV site found at 3649
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added Start and Stop codons


Source

Source = Addgene 48873.

References