Coding
Part:BBa_J428061:Design
Designed by: Vinoo Selvarajah Group: 2021 Engineering Committee (2022-06-07)
Cas_variants_hLwCas13a
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2830
Illegal PstI site found at 2851 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2830
Illegal PstI site found at 2851 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2830
Illegal BglII site found at 1286
Illegal BglII site found at 1496
Illegal BglII site found at 1763
Illegal BglII site found at 2528 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2830
Illegal PstI site found at 2851 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2830
Illegal PstI site found at 2851 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added stop codon (taa).
Source
Source = Addgene 90097.