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Part:BBa_J42043:Design

Designed by: Steve Selinsky   Group: iGEM06_Michigan   (2006-10-30)


araB complete recomb. plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 1202
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 1231
    Illegal XhoI site found at 1249
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal AgeI site found at 73
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 148


Design Notes

The flanking homologous regions and drug resistance gene frame a six restriction site poly-linker. Adjacent restriction sites had to be chosen for compatible double digestion to facilitate proper insert orientation.


Source

The polylinker, as well as the front and rear end of the AraB homologous regions come from annealed oligonucleotides. The Kanamycine gene extracted, via PCR, from the pDK11 plasmid

References