Generator

Part:BBa_J36848:Design

Designed by: Perry Tsai   Group: iGEM2006_Harvard   (2006-10-30)


Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin wild-type + His6tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 432
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 474
    Illegal AgeI site found at 525
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is no spacer nucleotide between the end of the device and the SpeI restriction site.

Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. This does not apply between R0010 and B0034 where there are still two spacer nucleotides, and after B0034 where there is still one spacer nucleotide, because R0010 and B0034 are standard bioBrick parts.

Source

Assembly of BBa_J04500 and BBa_J36844

References