T7

Part:BBa_J34811:Design

Designed by: Alexandra Choutko   Group: iGEM06_ETHZ   (2006-10-26)

T7 polymerase with stop codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1850
    Illegal NgoMIV site found at 1969
    Illegal NgoMIV site found at 2584
    Illegal NgoMIV site found at 2593
    Illegal AgeI site found at 2144
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References

Simon L. Dove and Ann Hochschild, Conversion of the v subunit of Escherichia coli RNA polymerase into a transcriptional activator or an activation target, Genes and development 12:745–754

James C. Hu, Michael G. Kornacker, and Ann Hochschild, Escherichia coli One- and Two-Hybrid Systems for the Analysis and Identification of Protein Protein Interactions, Methods 20, 80-94 (2000)