Reporter

Part:BBa_J33203:Design

Designed by: Chris French   Group: iGEM06_Edinburgh   (2006-10-13)


E. coli ars promoter, arsR gene and lacZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since this construct includes the arsR gene encoding the ArsR repressor which regulates the ars promoter, it requires no other promoter-specific functions. However, it will only give LacZ activity in a host expressing the E. coli lacZ-delta-M15 gene.


Source

The ars promoter and arsR gene are derived from E. coli JM109 chromosomal DNA. The lacZ' minigene is derived from E. coli BL21 chromosomal DNA, since JM109 does not possess an intact lacZ gene.

References