Generator

Part:BBa_J3105

Designed by: Davidson and Missouri Western   Group: iGEM06_Davidson   (2006-08-01)


pBad-RBS-HixC-TetB-HixC-TT-RE

This part contains the backwards coding region for the tetracycline resistance gene flanked by Hix C sites. If the Hin( BBaJ31000) ot the Hin-LVA (BBa_J31001) gene with a promoter and terminator, the backwards TetR gene will be inverted so that tetracyline resistance will be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1238
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1092
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 538
    Illegal NgoMIV site found at 698
    Illegal NgoMIV site found at 1066
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/hin
Parameters
None