Part:pSB1A7

Transcriptionally insulated high copy BioBrick plasmid

pSB1A7 was constructed by adding double terminators (BBa_B0015) before the BioBrick prefix and after the BioBrick suffix in pSB1A3. The added terminators block read-through transcription coming from the vector backbone into the BioBrick MCS.

pSB1A7 was promoted from BBa_J31009 by Randy on 11/16/2006.

pSB1A8 is a newer, modified version of pSB1A7 with the BsaI site in the ampR gene removed (J119043).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2411
  Illegal XbaI site found at 2426
  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2411
  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  Illegal NotI site found at 8
  Illegal NotI site found at 2417
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2411
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2411
  Plasmid lacks a suffix.
  Illegal XbaI site found at 2426
  Illegal SpeI site found at 1
  Illegal PstI site found at 15
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1312