Intermediate

Part:BBa_J23026:Design

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-07-25)


[TraG][TT]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1604
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 79
    Illegal NgoMIV site found at 205
    Illegal NgoMIV site found at 388
    Illegal NgoMIV site found at 649
    Illegal NgoMIV site found at 696
    Illegal NgoMIV site found at 906
    Illegal NgoMIV site found at 1402
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1710


Design Notes

n/a


Source

[TraGr][TT] Digest pSB1A2-J23013 (EcoRI/SpeI, Neb2, Small ) Paste into pSB1AKG0-B0015 (EcoRI/XbaI, Neb2, Large 2062) Product is pSB1A2-J23026.

References