Cell

Part:BBa_J23016:Design

Designed by: Will Bosworth   Group: iGEM06_Berkeley   (2006-07-24)


F plasmid with trbC knockout, CmR (graveyarded)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

JL12 Forward oligo to knockout trbC-f: atgaagctgagtatgaaatctctggcagcactgctgatgatgctggtgCaggctggagctgcttcgaag JL13 Reverse oligo to knockout trbC-f: tcatttcccggaatctcctttccctgccagtaaatcatgagccactgggaaGtagccatggtccatat


Source

PCR JL12/JL13 on pKD3 (~1000bp, dPN1, Neb2) Electroporate into pKD46xpOX38

References