Plasmid

Part:BBa_J23004:Design

Designed by: John Anderson   Group: iGEM2006_Berkeley   (2006-06-23)

Plasmid for destroying biobrick restriction sites


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 957
    Illegal SpeI site found at 16
    Illegal PstI site found at 971
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 16
    Illegal PstI site found at 971
    Illegal NotI site found at 7
    Illegal NotI site found at 964
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 957
    Illegal SpeI site found at 16
    Illegal PstI site found at 971
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 957
    Illegal SpeI site found at 16
    Illegal PstI site found at 971
    Illegal AgeI site found at 667
    Illegal AgeI site found at 779
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2130


Design Notes

N/A


Source

pSB1A3-I13521
See Berkeley iGEM2006 construction file: JCA062306-Construction of pJ23004

References