Part:BBa_J23004

Plasmid for destroying biobrick restriction sites

This plasmid is used to destroy the XbaI or SpeI restriction sites of a biobricked part.

Inserting an XbaI/PstI fragment of a biobrick part into the SpeI/PstI sites destroys the XbaI site

Inserting an EcoRI/SpeI fragment of a biobrick part into the EcoRI/XbaI sites destroys the SpeI site

To construct restiction-site deficient variants of a part in a different pSB* plasmid, transfer the EcoRI/PstI fragment into similar sites.

This part describes the pSB1A3 variant of the plasmid

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1
  Illegal XbaI site found at 957
  Illegal SpeI site found at 16
  Illegal PstI site found at 971
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1
  Illegal SpeI site found at 16
  Illegal PstI site found at 971
  Illegal NotI site found at 7
  Illegal NotI site found at 964
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1
  Illegal XbaI site found at 957
  Illegal SpeI site found at 16
  Illegal PstI site found at 971
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1
  Illegal XbaI site found at 957
  Illegal SpeI site found at 16
  Illegal PstI site found at 971
  Illegal AgeI site found at 667
  Illegal AgeI site found at 779
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2130