Coding
Tc2bh

Part:BBa_J212006:Design

Designed by: Xing Yan   Group: Zhou Lab   (2012-02-03)

Exo2b catalytic domain fused with the cbh1 gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 406
    Illegal EcoRI site found at 1060
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 406
    Illegal EcoRI site found at 1060
    Illegal NheI site found at 2193
    Illegal NheI site found at 2320
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 406
    Illegal EcoRI site found at 1060
    Illegal BglII site found at 758
    Illegal BamHI site found at 1069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 406
    Illegal EcoRI site found at 1060
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 406
    Illegal EcoRI site found at 1060
    Illegal NgoMIV site found at 1960
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 244
    Illegal BsaI.rc site found at 1204


Design Notes

The endoglucanase gene exo2b was fused with the native gene cbh1 to facilitate the translocation of the foreign protein in the secretory pathway of Trichoderma reesei and avoid degradation.

Source

Metagenomic fosmid library of a biogas digester fed with pig manure and rice straw

References