Plasmid_Backbone

Part:BBa_J18964:Design

Designed by: Sean Sleight   Group: Affiliates   (2013-06-18)


pGA3K4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4711
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4711
    Illegal NheI site found at 1384
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4711
    Illegal BglII site found at 4726
    Illegal BamHI site found at 1
    Illegal XhoI site found at 16
    Illegal XhoI site found at 177
    Illegal XhoI site found at 1020
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4711
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4711
    Illegal AgeI site found at 1470
    Illegal AgeI site found at 1793
    Illegal AgeI site found at 4610
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2498
    Illegal SapI site found at 4592


Design Notes

Inserted luxR and araC to be constitutively expressed in the reverse direction on the vector.


Source

Modified pGA3K3 from 2011 University of Washington iGEM team.

References