Part:BBa_J18926:Design

FRB(T2098L)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 225
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

• aa translation from human mTOR,

• mutation T2089L (verified against pC4-RHE from Ariad)

• GeneArt codon optimization for E. coli

• gene synthesis

Source

human mTOR (aa sequence)

gene synthesis