Plasmid_Backbone

Part:BBa_J179002:Design

Designed by: Jara Radeck   Group: Mascher Lab   (2013-12-20)


pBs4S: Bacillus subtilis vector, thrC integration, spec resistance


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4552
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4558
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4552
    Illegal BamHI site found at 4534
    Illegal XhoI site found at 1011
    Illegal XhoI site found at 1929
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4552
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4552
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4567
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2856
    Illegal SapI.rc site found at 605


Design Notes

This vector is compatible with pBS1C and pBS2E in one strain.


Source

This BioBrick is part of the LMU 2012 igem project BacillusBioBrickBox

For handling of B. subtilis vectors, please see here.

The transformation into B. subtilis is explained here.

References