Part:BBa_J176171:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQe65a5e7cecc980d8-partinfo-00000000-QINU UNIQe65a5e7cecc980d8-partinfo-00000001-QINU Hong Kong_HKUST iGEM 2013 planned to use this plasmid as backbone for transfecting in mammalian cells. However, we found that this plasmid is degradable at a high temperature during polymerase chain reaction.

Figure 1: Degradation of BBa_176171 plasmid at temperature above 50°C after denaturation step of polymerase chain reaction. BBa_176171 and pEGFP-N1 were placed in ddH2O and ran polymerase chain reaction denaturation step at 98°C for 30 seconds and placed in a range of temperature from 50°C to 59°C for 30minutes. For controls, BBa_176171 and pEGFP-N1 were stored in room temperature (24°C) and 4°C for 30minutes respectively. The plasmids were ran on Gel Red pre-stained 0.8% agarose gel using 140V for 25minutes. 0.5uL of GeneRuler 1kb ladder was used.