Protein_Domain
PLrigid

Part:BBa_J176131:Design

Designed by: Karmella Haynes   Group: Haynes Lab   (2011-10-28)

PLrigid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 20
    Illegal PstI site found at 35
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • The peptide sequence is repetitive [EAAAR][EAAAR][EAAAR][EAAAR] (Yan et al., 2007). In order to reduce repetition at the DNA sequence level, human-optimazed codons were distributed randomly along the sequence. This was done using a permutation tool I found online, along with some tedious manual text editing to create more variation where the sequence still looked too repetitive
  • De novo DNA synthesis was provided by Genscript


Source

TBA

References

  1. Yan W, Imanishi M, Futaki S, Sugiura Y. (2007) alpha-Helical Linker of an Artificial 6-Zinc Finger Peptide Contributes to Selective DNA Binding to a Discontinuous Recognition Sequence. Biochem. 46: 8517-8524