Regulatory
HPK

Part:BBa_J176028

Designed by: Karmella Haynes   Group: Haynes_Lab   (2011-09-29)

HPK

Constitutive human phosphoglycerate kinase promoter


Usage and Biology

Comparison of human promoters in U2OS cells
  • "Medium" strength expression compared to CMV
  • Constitutive (does not require induction)

The human phosphoglycerate kinase (HPK) promoter drives expression at a level intermediate to the ubiquitin C promoter (Ubc) and the popular cytomegalovirus promoter (CMV) in mammalian cells.

REFERENCES

  • Will be added soon


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 116
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
chassismammalian cells