Generator

Part:BBa_J153003

Designed by: Daniel Camsund / Hsinho Huang   Group: Lindblad Lab   (2012-02-03)

Ptrc1O-driven GFP reporter

Uses the strong Ptrc1O promoter (Part:BBa_J153001) to generate untagged GFPmut3b (Part:BBa_E0040).

Usage and Biology

This strong GFP generator was used to test for the replication of the broad-host-range shuttle vector pPMQAK1 (Part:BBa_J153000) in various cyanobacteria (Huang etal 2010) [http://www.ncbi.nlm.nih.gov/pubmed/20236988]. This reporter carried on pPMQAK1 has also been used as a reference construct for free cytoplasmic GFP in cyanobacterial protein localization studies (Camsund etal 2011) [http://www.ncbi.nlm.nih.gov/pubmed/21204939].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 733


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Categories
Parameters
None