Generator
Part:BBa_J153003
Designed by: Daniel Camsund / Hsinho Huang Group: Lindblad Lab (2012-02-03)
Ptrc1O-driven GFP reporter
Uses the strong Ptrc1O promoter (Part:BBa_J153001) to generate untagged GFPmut3b (Part:BBa_E0040).
Usage and Biology
This strong GFP generator was used to test for the replication of the broad-host-range shuttle vector pPMQAK1 (Part:BBa_J153000) in various cyanobacteria (Huang etal 2010) [http://www.ncbi.nlm.nih.gov/pubmed/20236988]. This reporter carried on pPMQAK1 has also been used as a reference construct for free cytoplasmic GFP in cyanobacterial protein localization studies (Camsund etal 2011) [http://www.ncbi.nlm.nih.gov/pubmed/21204939].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 733
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Categories
Parameters
None |