Regulatory
Part:BBa_J153001:Design
Designed by: Daniel Camsund / Hsinho Huang Group: Lindblad Lab (2012-02-03)
Ptrc1O
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
Ptrc1O, or the trc promoter, was extracted from the pTrc99A plasmid using PCR (Huang etal 2010, Brosius etal 1985).
References
Brosius, J., Erfle, M. and Storella, J. (1985) Spacing of the -10 and -35 Regions in the Tac Promoter - Effect on Its Invivo Activity. Journal of Biological Chemistry, 260, 3539-3541.
Huang, H.H., Camsund, D., Lindblad, P. and Heidorn, T. (2010) Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology. Nucleic Acids Research, 38, 2577-2593.