Regulatory

Part:BBa_J119439

Designed by: Todd Eckdahl   Group: Eckdahl Lab   (2019-07-18)


BCL-2 Promoter G-quadruplex and i-Motif

The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. The G-quadruplex is shown on the coding strand in this sequence. This places the i-motif sequence on the template strand. If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either orientation (i-motif on the coding strand or G-quadruplex on the coding strand). The i-motif/G-quadruplex-forming sequence comes from the human BCL-2 promoter


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None