Regulatory
Part:BBa_J119405:Design
Designed by: Lindsay Doolan and the fall 2016 Missouri Western Genetics class Group: Eckdahl Lab (2017-03-24)
(pSB1A2-BR) Deletion of the original -35 sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The original -35 region, was deleted, but transcription was not terminated, the sequence just shifted, filling in a new sequence for the -35 region.
Source
Part-sequence only (35 bp) AAGAATACCTTGAAGAAAGGAGGTATAATCCGTCG