Measurement

Part:BBa_J119401:Design

Designed by: Avery Umphreys   Group: Eckdahl Lab   (2016-07-18)


tCloneTet+PDB riboswitch with P5 and BD2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 83
    Illegal NheI site found at 458
    Illegal NotI site found at 89
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 83
    Illegal BamHI site found at 604
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 83
    Illegal XbaI site found at 98
    Illegal NgoMIV site found at 630
    Illegal NgoMIV site found at 998
    Illegal NgoMIV site found at 1173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BD2 was inserted to the plasmid via Decoupled Golden Gate Assembly. Using iPCR, all of the J100255 plasmid was amplified, besides BD18.


Source

J100255, original tClone tet+PDB Riboswitch, is located in Campbell M Lab. BD2, located in Eckdahl Lab, is part number J119025.

References