Measurement
Part:BBa_J119401:Design
Designed by: Avery Umphreys Group: Eckdahl Lab (2016-07-18)
tCloneTet+PDB riboswitch with P5 and BD2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 83
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 83
Illegal NheI site found at 458
Illegal NotI site found at 89 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 83
Illegal BamHI site found at 604 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 83
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 83
Illegal XbaI site found at 98
Illegal NgoMIV site found at 630
Illegal NgoMIV site found at 998
Illegal NgoMIV site found at 1173 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
BD2 was inserted to the plasmid via Decoupled Golden Gate Assembly. Using iPCR, all of the J100255 plasmid was amplified, besides BD18.
Source
J100255, original tClone tet+PDB Riboswitch, is located in Campbell M Lab. BD2, located in Eckdahl Lab, is part number J119025.