Measurement

Part:BBa_J119400:Design

Designed by: Avery Umphreys   Group: Eckdahl Lab   (2016-07-18)


tCloneTet+PDB riboswitch with P5 and BD10


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 79
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 79
    Illegal NheI site found at 454
    Illegal NotI site found at 85
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 79
    Illegal BamHI site found at 600
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 79
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 79
    Illegal XbaI site found at 94
    Illegal NgoMIV site found at 626
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BD10 was inserted via Decoupled Golden Gate Assembly. iPCR amplified everything on the J100255 plasmid, besides BD18.


Source

J100255 is the original tClone Tet+PDB Riboswitch and it is from Campbell M Lab. J119026, located in Eckdahl Lab, includes the BD10 C Dog RBS.


References