Part:BBa_J119385

Theophylline Riboswitch - tetracycline resistance protein (BbsI site removed)

This device uses a synthetic riboswitch to control tetracycline resistance. This riboswitch is modified from riboswitch D as described in the paper by Topp et al. In the presence of theophylline, the transcription of the TetA gene occurs, but in the absence of theophylline, very little transcription takes place. This part contains a modified T5 promoter, which, in the absence of cymR, acts as a strong constitutive promoter. The ribosomal binding site is contained in the riboswitch. A BbsI site was removed from the TetA gene.

Reference: Shana Topp, Colleen M. K. Reynoso, Jessica C. Seeliger, Ian S. Goldlust, Shawn K. Desai, Dorothée Murat, Aimee Shen, Aaron W. Puri, Arash Komeili, Carolyn R. Bertozzi, June R. Scott, and Justin P. Gallivan. Synthetic Riboswitches That Induce Gene Expression in Diverse Bacterial Species. Applied and Environmental Microbiology, 76:23, 7881-7884. December 2010.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 306
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 452
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 478
  Illegal NgoMIV site found at 846
  Illegal NgoMIV site found at 1006
  Illegal AgeI site found at 100
  
• 1000
  COMPATIBLE WITH RFC[1000]