DNA

Part:BBa_J119142:Design

Designed by: Caleb J. Carr   Group: Eckdahl Lab   (2013-05-08)


Part for inserting modified D-Dogs for Golden Gate Assembly using BsgI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 660
    Illegal AgeI site found at 772
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 48
    Illegal BsaI.rc site found at 6


Design Notes

To insert this part, oligonucleotides were designed to replace the sequence from the BBA prefix to a rogue BsiWI site about 110 into the mRFP gene. The new part now has a functional promoter leading a non-functional C-Dog, followed by mRFP.

Source

Part is mostly nonsense except for restriction enzyme recognition sequence.

References