Regulatory

Part:BBa_J119132

Designed by: MWSU Genetics Lab Fall 2012 2:00   Group: Eckdahl Lab   (2013-01-07)


pTac with mutations

This part was designed to see the effects of mutations on a functional promoter. Golden Gate Assembly was used to insert the mutations into Part J100091 at the double terminator sites. This promoter sequence is without the -10 element and the -35 element is present. Golden Gate Assembly with annealed oligos with J100091 as the receiving plasmid and Bsa I sticky ends. This promoter inserted at the double terminator region. There was a loss of function with this promoter. The RFP was unable to be turned on.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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