Regulatory

Part:BBa_J119123:Design

Designed by: Caleb J. Carr   Group: Eckdahl Lab   (2012-09-17)


Promoter-tryptophan, Ptrp, repressible promoter of the tryptophan operon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 23
    Illegal SpeI site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part was created using only primers, though is a sequence-identical bio-bricked part of the source. Including the biobrick ends, part is 91bp long.


Source

Created using primers alone, though copied from a sequence that exists in E. coli.

References