Other
Part:BBa_J119084:Design
Designed by: David Carr Group: Eckdahl Lab (2012-03-09)
GFP Node for GGA/HPP 2.0
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal PstI site found at 746 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal PstI site found at 746 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal PstI site found at 746 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal PstI site found at 746 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 740
Design Notes
This part contains four Type II restriction enzymes (BsaI, BbsI, PstI, and EcoRI) cutting sights which flank a "Gene of Interest", which in this case is a GFP reporter.
Source
de novo