Reporter

Part:BBa_J119068:Design

Designed by: Eric Sawyer   Group: Eckdahl Lab   (2012-02-02)

RFPrev-RBSrev-pT7rev-pLacI-RBS-T7 RNAP (2SAT mutant 1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 35
    Illegal AgeI site found at 147
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 911
    Illegal BsaI.rc site found at 862


Design Notes

The frameshift mutation is of the form (a’ or b) and (a or c’).


Source

Existing part and synthetic oligos.

References