Plasmid_Backbone

Part:BBa_J119048:Design

Designed by: A. Malcolm Campbell and Todd Eckdahl   Group: Eckdahl Lab   (2012-01-23)


pSB4A8


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 3629
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3629
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3635
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3629
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3629
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3629
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3644
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

BsaI site removed from amplicillin resistance gene. Contains stuffer piece of GCATGCATGCAT to be removed when cloning parts.


Source

de novo DNA synthesis

References