Regulatory

Part:BBa_J107109:Design

Designed by: Susanna Zucca   Group: Magni Lab   (2014-12-12)

LuxR-repressible promoter library member


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Inspired by the Egland and Greenberg paper ( http://jb.asm.org/cgi/content/abstract/182/3/805 ) and by the Anderson promoter collection sequences.

Source

PCR with Taq polymerase and degenerate oligonucleotides.

References

Zucca S, Pasotti L, Politi N, Casanova M, Mazzini G, Cusella De Angelis MG, Magni P: Multi-faceted characterization of a novel LuxR-repressible promoter library for Escherichia coli. ADD LINK