Reporter

Part:BBa_J10069:Design

Designed by: Malcolm Campbell   Group: iGEM2005   (2012-01-10)


EPIC Firefly Luciferase and LRE P. pyralis + BD18


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2624
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2625
    Illegal PstI site found at 2639
    Illegal NotI site found at 2632
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 2577
    Illegal BamHI site found at 945
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2625
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2625
    Illegal PstI site found at 2639
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal SapI.rc site found at 2281


Design Notes

removed RBS from J119024


Source

from registry parts, synthesized de novo

References