Reporter

Part:BBa_J10063:Design

Designed by: Malcolm Campbell   Group: iGEM2005   (2012-01-10)


Green Firefly Luciferase and LRE L. Cruciata (E. coli optimised)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2060
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 951
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 900
    Illegal AgeI site found at 2602
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 332
    Illegal SapI.rc site found at 2116


Design Notes

Wanted to allow users to use their favorite promoter.


Source

from registry, synthesized de novo.

References