Measurement

Part:BBa_J10060:Design

Designed by: Malcolm Campbell   Group: iGEM2005   (2012-01-10)


pBad+BD18+RFP_withoutATG no scars


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 770
    Illegal AgeI site found at 882
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This had to be synthesized de novo to get rid of E1010 start codon as well as all scars.


Source

registry parts

References