Part:BBa_J10058:Experience

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Applications of BBa_J10058

Lambert_GA 2019 used this part in experiments to characterize the effect of different strengths of promoters and ribosomal binding sites on expression. The data obtained can aid in the design of constructs by giving information about controlling expression.

Lambert_GA 2019 Characterization

Characterization of Strong Promoter (BBa_J23119) and Strong RBS (BBa_B0034)

Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10058 would result in the most amount of expression out of the tested combinations, but this part, along with BBa_J10056 and BBa_J10057, all shared the most expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths.

Figure 1: The highlighted points in yellow represent BBa_J10058 (strong promoter/ strong RBS) compared to BBa_J10057 (strong promoter/ medium RBS) and BBa_J10056 (strong promoter/ weak RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter.

Figure 2: The highlighted points represent the BBa_J10058 composite part (strong promoter/ strong RBS). As the promoter strength increases, the expression increases as well.

The graphs above show that RBS strength has a negligible effect on expression, whereas increased promoter strength increases expression.

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