Reporter

Part:BBa_J100435:Design

Designed by: Tatianna Travieso   Group: Campbell M Lab   (2018-07-12)


actClone 2 Red


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1556
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1556
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1556
    Illegal AgeI site found at 1429
    Illegal AgeI site found at 1541
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 806
    Illegal BsaI.rc site found at 12
    Illegal SapI site found at 719


Design Notes

modeled after repClone 2 Red (J100333). RBSs needed to be less similar than those in the original actClone. Mutations in RFP were needed to prevent RBS and anti-RBS binding, mutated RFP was modeled after the sequence in repClone V2 (J100342)


Source

(J100204)

References