Reporter

Part:BBa_J100435

Designed by: Tatianna Travieso   Group: Campbell M Lab   (2018-07-12)


actClone 2 Red

modified actClone (J100204) with basic strong RBSs replacing the Cdog RBSs, the transcriptional terminator removed, and BsaI sites that cut out the GFP along with the plasmid's original reverse promoter and reverse RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1556
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1556
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1556
    Illegal AgeI site found at 1429
    Illegal AgeI site found at 1541
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 806
    Illegal BsaI.rc site found at 12
    Illegal SapI site found at 719


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