Plasmid

Part:BBa_J100422:Design

Designed by: Morgan Klein   Group: Campbell M Lab   (2018-06-21)


pClone mScarlet with weak promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 930
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 930
    Illegal BamHI site found at 876
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 930
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 930
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 804


Design Notes

We added the correct sticky ends to the J100329 promoter


Source

It comes from the original pClone mScarlet plasmid sequence and the J100329 sequence

References