Composite

Part:BBa_J100416:Design

Designed by: Caroline Willis, Tatianna Travieso   Group: Campbell M Lab   (2018-06-21)


T7 Consensus Promoter in pClone Red


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1437
    Illegal AgeI site found at 1549
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The paper referenced earlier discussed studies that found an "initiation domain" and a "binding domain" from -12 to +5, both of which were essential for successful transcription and translation.


Source

"T7 promoter essential for promoter activity in vivo" by Richard A. Ikeda et al. (1992), https://www.ncbi.nlm.nih.gov/pubmed/1598210 Consensus sequence, https://parts.igem.org/Promoters/Catalog/T7Promoters/Catalog/T7

References